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投稿e-mail: jbnuns_sub@bnu.edu.cn

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标题

通过扩大非甲羟戊酸途径以实现S-柠檬烯在E.coli中的生物合成

作者

王建辉 艾莹 杨冬

机构

北京师范大学生命科学学院,北京市基因工程药物与生物技术重点实验室

摘要

单萜烯系香叶基焦磷酸(GPP)环化后的产物。其在食品化工医药能源等领域具有广泛的应用。目前,单萜烯主要从植物精油中纯化获得,因而产量受到限制。代谢工程是近来的新兴生物工程技术。通过改造代谢通路,可以实现在微生物中生产各类重要的产物。要实现单萜烯的微生物生产,除了要引入相应的萜烯合成酶外,还需要提高其上游产物,即异戊二烯焦磷酸(IPP)和GPP的供给。在本项工作中,我们首次通过放大E.coli的非甲羟戊酸途径,增加了IPP前体的合成,再结合引入GPP合酶及S-柠檬烯合成酶,实现了在细菌内生物合成S-柠檬烯。本工作为未来通过微生物发酵方法生产各类单萜烯产物提供了基础。

关键词

单萜烯; 柠檬烯; 非甲羟戊酸; 甲基赤藓糖-4-磷酸途径; 大肠杆菌; 异戊二烯

引用

王建辉, 艾莹, 杨冬. 通过扩大非甲羟戊酸途径以实现S-柠檬烯在E.coli中的生物合成.[J]. 北京师范大学学报(自然科学版),2016,52(2):167-171.

基金

国家自然科学基金资助项目(31070682); 教育部留学回国人员科研启动基金资助项目; 霍英东教育基金会资助项目

分类号

Q812

DOI

10.16360/j.cnki.jbnuns.2016.02.010

Title

S-Limonene production in engineered E. coli with an amplified MEP pathway

Author

ZHAO Lin, ZHANG Quanguo, ZHANG Dayong

Affiliations

Beijing Normal University, College of Life Sciences, Gene Engineering and Biotechnology Beijing Key Laboratory

Abstract

Monoterpenes derived from geranyl pyrophosphate (GPP) have been used as food flavors, pharmaceuticals, precursors for chemical synthesis and biofuels. As extraction from essential oils is still the most commonly used method for their production, the supply of monoterpenes is limited by natural resources. Recently, metabolic engineering has been developed to re-design microbes to synthesize novel compounds, including monoterpenes. To achieve this, it is essential to increase the supply of two precursors IPP and GPP. In this work, we amplified the none-mevalonate pathway (MEP pathway) in E. coli in order to increase intracellular IPP concentration. This combined with the introduction of GPP synthase (GPPS) and S-limonene synthase led to the successful production of S-limonene in E. coli. Our results provide the foundation for manufacturing important monoterpenes in bacteria.

Key words

Escherichia coli; experimental evolution; responses to seletion; rugged landscape

cite

ZHAO Lin, ZHANG Quanguo, ZHANG Dayong. S-Limonene production in engineered E. coli with an amplified MEP pathway [J]. Journal of Beijing Normal University(Natural Science), 2016,52(2):167-171.

DOI

10.16360/j.cnki.jbnuns.2016.02.010

Copyright © 2014 Journal of Beijing Normal University (Natural Science)
Designed by Mr. Sun Chumin. Email: cmsun@mail.bnu.edu.cn