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投稿e-mail: jbnuns_sub@bnu.edu.cn

主编e-mail: jbnuns_eic@bnu.edu.cn

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标题

大丽轮枝菌拮抗芽孢菌株的分离、鉴定及 两株菌抑菌特性研究

作者

赵君洁 曾卫军 李艳红 葛风伟 杜 钰 袁琳琳 赵歉歉 王 敏 谢红桃 白若翔 韩生成 赵和平 赵惠新

机构

新疆师范大学生命科学学院, 新疆特殊环境物种保护与调控生物学实验室; 北京师范大学生命科学学院, 抗性基因资源与分子发育北京市重点实验室

摘要

从新疆和硕连作棉田根际土壤中分离到146株芽孢细菌.29株对大丽轮枝菌(Verticillium dahliae Kleb)具 有拮抗作用的菌株中有9株拮抗性极强.经鉴定,9株拮抗性极强的菌株中有2株属短小芽孢杆菌(Bacillus pumilus),7 株与枯草芽孢杆菌(B.subtilis)相近.抑菌特性实验表明: J-02 和 J-15 的抑菌高峰分别出现在衰亡期和稳定期,对 pH 的耐受范围分别为 5~9 和 7~9; J-02 的热稳定性较好, J-15对温度较敏感,但 100℃ 处理后仍有抑菌活性; J-02 能够抑制 大丽轮枝菌芽管伸长形成菌丝, J-15 可导致大丽轮枝菌菌丝畸形,失去产孢能力.盆栽实验表明, J-02 和 J-15 这2株拮抗 菌对棉花黄萎病有明显防治作用.综合结果表明J-02和J-15均有作为棉花黄萎病生防菌的潜能.

关键词

棉花黄萎病; 大丽轮枝菌; 拮抗作用; 芽孢杆菌

引用

赵君洁 曾卫军 李艳红 葛风伟 杜 钰 袁琳琳 赵歉歉 王 敏 谢红桃 白若翔 韩生成 赵和平 大丽轮枝菌拮抗芽孢菌株的分离、鉴定及 两株菌抑菌特性研究.[J]. 北京师范大学学报(自然科学版),2017,53(3): 294-300.

基金

新疆维吾尔自治区高校科研计划科学研究重点资助项目(20140705081400714); 新疆维吾尔自治区教育厅重点实验室“新疆特殊环境物种多样性应用与调控重点实验室”资助项目(XJTSWZ-2015-01); 新疆师范大学博士启动基金资助项目(XJNUBS1416); 抗性基因资源与分子发育北京市重点实验室开放基金资助项目(2015GD03)

分类号

S435.621.2

DOI

10.16360/j.cnki.jbnuns.2017.03.009

Title

Isolation and identification of antagonistic Bacillus spp. against Verticillium dahliae: the antibacterial properties of two strains

Author

ZHAO Junjie ZENG Weijun LI Yanhong GE Fengwei DUYu YUAN Linlin ZHAO Qianqian WANG Min XIE Hongtao BAI Ruoxiang HAN Shengcheng ZHAO Heping ZHAO Huixin

Affiliations

Key Laboratory of Special Species Conservation and Regulatory Biology of Xinjiang, College of Life Sciences, Xinjiang Normal University; Key Laboratory of Gene Resource and Molecular Development of Beijing, College of Life Sciences, Beijing Normal University

Abstract

Cotton Verticillium wilt is the most serious fungal disease in Chinese cotton production, screening for its control for stable and high yields is of great significance in China. We have isolated antagonistic Bacillus strains against Verticillium dahliae from healthy continuously-cropping cotton rhizosphere soil in Xinjiang. A total of 146 strains were obtained and 29 strains showed varied degrees of antagonism to Verticillium dahliae. Of these strains J-02 and J-29 belonged to Bacillus pumilus and the remainder are similar to B.subtilis. We found that the strongest inhibition of J-02 on Verticillium dahliae was in decline phase, J-15 instable phase and inhibition was stable in the pH range from 5~9 for J-02 and 7~9 for J-15 respectively. Strain J-02 was found to be more resistant to heat, J-15 was sensitive to temperature, but there was still an inhibition zone after 100℃ treatment J-02 inhibited the germ tube of Verticillium dahilae e-longates to form mycelium. J-15 caused mycelium of Verticillium dahliae to be deformed, and to fail to produce spores. In pot experiments, J-02 and J-15 showed acertain protective effect on cotton Verticillium wilt. In conclusion, strains J-02 and J-15 have potentials as biocontrol vectors against cotton Verticillium wilt.

Key words

cotton Verticillium wilt; Verticillium dahliae; antagonism; Bacillus

cite

ZHAO Junjie ZENG Weijun LI Yanhong GE Fengwei DUYu YUAN Linlin ZHAO Qianqian WANG Min XIE Hongtao BAI Ruoxiang HAN Shengcheng ZHAO Heping ZHAO Huixin. Isolation and identification of antagonistic Bacillus spp. against Verticillium dahliae: the antibacterial properties of two strains [J]. Journal of Beijing Normal University (Natural Science), 2017,53(7): 294-300.

DOI

10.16360/j.cnki.jbnuns.2017.03.009

Copyright © 2014 Journal of Beijing Normal University (Natural Science)
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